Bimetallic MnCo oxide nanohybrids prepared from Prussian blue analogue for application as impedimetric aptasensor carrier to detect myoglobin
CHEMICAL ENGINEERING JOURNAL(2020)
Abstract
Abstract The accurate detection of myoglobin (Myo) in human serum plays a crucial role in early diagnosis of acute myocardial infarction. This work presents a novel impedimetric aptasensor for sensitive and selective detection of Myo, in which bimetallic MnCo oxide nanohybrids (represented by MnOxCoOy) were used as platforms for anchoring Myo aptamer strands. A series of MnOxCoOy nanohybrids were prepared from bimetallic MnCo-based Prussian blue analogue (PBA) nanocubes via calcination at different temperatures under N2 atmosphere. Basic characterizations revealed that homogeneously-distributed MnOx and CoOy nanoparticles were achieved at 500 °C (MnOxCoOy,500) calcination, which immensely enhanced the electrochemical conductivity in addition to boosting the aptamer immobilization. Compared with the pristine MnCo-PBA-based aptasensor, the MnOxCoOy,500-based aptasensor exhibited higher sensitivity and selectivity for Myo detection, with lower detection limit of 0.56 fg·mL-1 (0.034 fM) in a wide dynamic range of 0.01∼2000 pg·mL-1, which is the most sensitive among similar previously reported Myo sensors. Moreover, the constructed MnOxCoOy,500-based aptasensor displayed good stability, excellent reproducibility and acceptable applicability in human serum. The proposed strategy establishes a remarkable platform for the construction of aptasensor that is feasible for the Myo detection in a real human serum sample.
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Key words
Myoglobin detection,Prussian blue analogue,Bimetallic MnCo oxides,Electrochemical impedance spectroscopy,Aptasensor
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