Emulsion PCR Amplification of DNA Libraries with Degenerate Central Regions for Aptamer Selection

V. E. Kuznetsova,V. E. Shershov, R. A. Miftahov, S. A. Lapa,A. S. Zasedatelev,E. N. Timofeev,A. V. Chudinov

Russian Journal of Bioorganic Chemistry（2020）

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摘要

For emulsion PCR amplification of oligonucleotide combinatorial libraries the conditions for the stable generation of water-in-oil emulsions with drops of optimal size were found, which included the use of 3% ABIL EM 180 silicone emulsifier in the presence of 0.5% Triton Х100 in mineral oil and controlled mechanical mixing.

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关键词

ABIL EM 180,aptamer,emulsion PCR,ePCR,SELEX

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