Microsphere-Embedded Fibroblast Aggregates Showing Prolonged Survival
JOURNAL OF BIOMATERIALS AND TISSUE ENGINEERING(2019)
摘要
Fibroblast aggregates in the size range of a few hundred micrometers were prepared from a dissociated monolayer culture and fibroin microspheres under non-adhesion conditions. Transmission electron micrograph revealed the presence of fibroin microspheres entrapped in the aggregates. Microsphere-embedded 3T3 cell aggregates showed an increase in glucose consumption and survival. Fibroin microspheres also prolonged the survival of human dermal fibroblasts cultured under three-dimensional conditions. A nucleoside incorporation assay revealed that only a few cells in the aggregates were proliferating. Accordingly, fibroblast aggregates with fibroin microspheres can be a candidate having extended shelf life for grafting.
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关键词
Microsphere,Fibroblast Culture,Aggregate,Prolonged Survival,Suspension
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