A porphyrin-pyranine dyad for ratiometric fluorescent sensing of intracellular pH
Journal of Photochemistry and Photobiology A: Chemistry(2020)
摘要
The FRET-based porphyrin-pyranine dyad 1 exhibited sensitive and reversible emission ratio (F660/F435) response in a dynamic pH range of 2.6–8.6, and thus was applied for pHi calibration by fluorescence ratio imaging in living cells.
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关键词
Porphyrin,Pyranine,pH sensor,Ratiometric fluorescent sensor,Fluorescenceresonance energy transfer
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