Highly Sensitive Sensor Based On Pt@Mno2/Rgo Nanosheets As A Platform For Real-Time Monitoring Cellular Ros And Its Application In Diverse Cancers

Reactive oxygen species (ROS) has sparked a huge interest since its vital role in various biological processes, especially in tumorigenesis. As a target for researches on catching cancer earlier, a sensitive method to monitor slight changes of ROS in living cells is quite important. This study presented a facile way for preparation of manganese dioxide/reduced graphene oxide nanosheets with high loading platinum nanoparticles, and developed a high-performance sensor based on PtNPs@MnO2/rGO nanocomposites for monitoring cellular responses upon oxidative stress. This unique nanostructure was characterized by transmission electron microscopy (TEM), EDX mapping and X-ray diffraction (XRD). By taking advantages of excellent electrochemical properties and large surface of the obtained nanocomposites, an ultra-sensitive sensor, owning a detection limit of 1.3 nM and a wider linear range from 0.05 mu M to 7 mM, has been successfully applied to monitor slight ROS changes in three types of living cancer cells in real time. This work not only provides a powerful tool for further study on the role of ROS in carcinogenesis, but also makes a valuable contribution to screen early stage cancer more effectively in near future. (C) 2020 The Electrochemical Society ("ECS"). Published on behalf of ECS by IOP Publishing Limited.