Ion torrent-based nasopharyngeal swab metatranscriptomics in COVID-19.

Gubio S Campos,Silvia I Sardi,Melissa B Falcao,Emilia M M A Belitardo,Danilo J P G Rocha,Carolina A Rolo,Aline D Menezes,Carina S Pinheiro,Rejane H Carvalho,João P P Almeida,Eric R G R Aguiar,Luis G C Pacheco

Journal of Virological Methods（2020）

引用 11|浏览53

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摘要

•Detection of a SARS-CoV-2 genome through metatranscriptome next-generation sequencing.•Direct metatranscritptome sequencing from nasopharyngeal swab of a COVID-19 patient.•Depletion of human rRNA and in-house bioinformatics strategy for Ion torrent sequencing.

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关键词

RT-qPCR,Ct,NGS,SNP

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