Exposure of Intestinal Epithelial Cells to 2-Fucosyllactose and CpG Enhances Galectin Release and Instructs Dendritic Cells to Drive Th1 and Regulatory-Type Immune Development
BIOMOLECULES(2020)
Abstract
Intestinal epithelial cells (IEC) release immunomodulatory galectins upon exposure to CpG DNA (mimicking bacterial triggers) and short-chain galacto- and long-chain fructo-oligosaccharides (GF). This study aims to investigate the immunomodulatory properties of 2 '-fucosyllactose (2 '-FL), a non-digestible oligosaccharide (NDO) abundantly present in human milk, using a co-culture model developed to study the crosstalk between IEC and innate and adaptive immune cells. IECs, co-cultured with alpha CD3/CD28-activated peripheral blood mononuclear cells (PBMC), were apically exposed to NDOs and CpG, washed and co-cultured with immature monocyte-derived dendritic cells (moDC). Subsequently, moDC were co-cultured with naive CD4+ T-cells. In the presence of CpG, both 2 '-FL or GF-exposed IEC enhanced Th1-type IFN gamma and regulatory IL-10 secretion of PBMCs, compared to CpG alone, while Th2-type IL-13 was reduced. Both NDOs increased IEC-derived galectin-3, -4, -9 and TGF-beta 1 of CpG-exposed IEC. Only galectin-9 correlated with all modified immune parameters and TGF-beta 1 secretion. MoDCs exposed to 2 '-FL and CpG-conditioned IEC instructed IFN gamma and IL-10 secretion by CD4+ T-cells, suggesting the development of a regulatory Th1 response. These results reveal that 2 '-FL and GF could contribute to the mucosal immune development by supporting the effect of microbial CpG DNA associated with the modulation of epithelial galectin and TGF-beta 1 secretion.
MoreTranslated text
Key words
1,2 '-fucosyllactose 2,non-digestible oligosaccharides 3,galectins 4,intestinal epithelial cells 5,dendritic cells 6,mucosal immunity
AI Read Science
Must-Reading Tree
Example
![](https://originalfileserver.aminer.cn/sys/aminer/pubs/mrt_preview.jpeg)
Generate MRT to find the research sequence of this paper
Chat Paper
Summary is being generated by the instructions you defined