Avenanthramide-C prevents amyloid formation of bovine serum albumin
BIOPHYSICAL CHEMISTRY(2020)
摘要
The misfolding of protein and its assembly into amyloid fibrils with a characteristic beta-sheet-rich secondary structure, cause a lot of illnesses. Polyphenols have been extensively studied as a class of amyloid inhibitors, whose effect depends on the position and number of hydroxyl groups around the flavone backbone. In this study, we used bovine serum albumin (BSA) as an amyloid model to test the anti-amyloid effects of Avenanthramide-C (Avn-C), a molecule with a long aliphatic linker between two aromatic rings. We used spectroscopy techniques like thioflavin T fluorescence and circular dichroism, to follow the beta-sheet-rich aggregates of BSA upon incubation at 68 degrees C. Our results demonstrated that Avn-C shows higher inhibitory effect on BSA oligomerization at micromolar concentrations, than Epigallocatechin gallate (EGCG) and Curcumin, proving for the first time, that Avn-C can serve as potential molecule in preventing protein aggregation.
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关键词
Avenanthramide C,Amyloid fibrils,BSA aggregation,beta-Sheet structure,Thioflavin-T
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