Saccharopolyspora Coralli Sp. Nov. A Novel Actinobacterium Isolated From The Stony Coral Porites

A novel Gram-stain-positive, aerobic, non-motile actinobacterium, designated strain E2A(T). was isolated from a coral sample and examined using a polyphasic taxonomic approach. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain E2A(T) formed a distinct phyletic lineage in the genus Saccharopolyspora and was closely related to S. cavernae CCTCC AA 2012022(T) (96.4%) and S. lacisalsi CCTCC AA 2010012(T) (95.3%). The isolate grew at 15-35 degrees C, pH 5-12 and in the presence of 1-16% (w/v) NaCl. The cell-wall diamino acid was meso-DAP. Major fatty acids identified were iso-C-15:0, iso-C(16:0 )and C-17:1 omega 8c. The predominant menaquinone was MK-9(H-4). The polar lipids detected were diphosphatidylglycerol. phosphatidylglycerol. phosphatidylcholine, phosphatidylethanolamine, phosphatidylmethylethanolamine, one unidentified glycolipid, one unidentified phospholipid and one unidentified aminolipid. The genomic DNA G+C content was 68.6 mol%. Based on the data from the poly-phasic taxonomic study reported here, strain E2A(T) represents a novel species within the genus Saccharopolyspora, for which the name Saccharopolyspora coralli sp. nov. is proposed. The type strain is E2A(T)=(JCM 31844(T)=MCCC 1A17150(T)).