Luteimonas Gilva Sp. Nov., Isolated From Farmland Soil

A Gram-stain-negative, rod-shaped bacterium, strain H23(T), was isolated from farmland soil sampled in Enshi City, Hubei Province, PR China. The isolate grew optimally at 28-32 degrees C, pH 8.0 and with 0.5% (w/v) NaCl. Based on the results of 16S rRNA gene sequence and phylogenetic analyses, strain H23(T) belonged to the genus Luteimonas with the highest degree of 16S rRNA gene sequence similarity to Luteimonas cucumeris Y4(T) (97.41%). The DNA G+C content was 65.88mol%. The average nucleotide identity and the Genome-to-Genome Distance Calculator results also showed low relatedness (below 95 and 70%, respectively) between strain H23(T) and type strains in the genus Luteimonas. Ubiquinone-8 was the predominant quinone. The major fatty acids were iso-C-15:0, iso-C-16(:0), iso-C-17(:0) and iso-C-17:1 omega 9c. Polar lipids were dominated by diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and unidentified phospholipids. Low digital DNA-DNA hybridization values, as well as physiological and biochemical differences, such as no casein hydrolysis, being catalase-negative, and tesing positive for cystine arylamidase, alpha-chymotrypsin and N-acetyl-beta-glucosaminidase, could distinguish strain H23(T) from its closely related species. Strain H23(T) is considered to represent a novel species in the genus Luteimonas, for which the name Luteimonas gilva sp. nov. is proposed, with strain H23(T) (=CCTCCAB 2019255(T)=KCTC 72593(T)) as the type strain.