New primers, taxonomic database and cut-off value for processing nxrB gene high-throughput sequencing data by MOTHUR

To analyse the diversity of Nitrospira with functional nxrB genes, we designed and optimized a new primer set, Nxr-f27/Nxr-r617, and constructed a database for taxonomic classification. We also propose a cut-off value for processing the high-throughput sequencing data with MOTHUR.