Novel bioanalytical method for the characterization of the immune response directed against a bispecific F(ab) fragment
BIOANALYSIS(2020)
Abstract
Aim: The work was aimed at developing a bioanalytical approach to identify immunogenic parts of a bispecific F(ab) fragment and to characterize the immune response seen in a preclinical study. Experimental: The bioanalytical method consists of a set of domain detection assays that use germlined variants of the drug. Results: The method demonstrated that anti-drug antibodies (ADAs) were predominantly directed against both antigen-binding sites of the drug. Conclusion: The method was capable to discriminate between ADAs directed against one of the antigen-binding sites, both sites or the constant domain, allowing for an estimation of the relative binding prevalence for these subunits. The developed approach provides a practical and robust solution for exploratory characterization of ADAs against multidomain biotherapeutics.
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Key words
ADA,anti-drug antibody,bispecific F(ab),DDA,domain detection assay,immunogenicity response characterization,MDB,multi-domain biotherapeutic
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