Fluorescent substrates for haloalkane dehalogenases: Novel probes for mechanistic studies and protein labeling.

Veronika Dockalova,Esther M Sanchez-Carnerero,Zuzana Dunajova,Eduardo Palao,Michaela Slanska,Tomas Buryska,Jiri Damborsky,Petr Klán,Zbynek Prokop

Computational and Structural Biotechnology Journal（2020）

引用 7|浏览23

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摘要

•The first fluorescent substrates for haloalkane dehalogenases.•Potent probes for kinetic analysis and high-throughput activity screening.•Provide high specificity and efficiency for protein labelling.•Probes for studying protein hydration and dynamics or markers for cell imaging.•Offer a unique possibility to directly label the enzyme active site.

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关键词

Haloalkane dehalogenase,Fluorescent substrate,Enzyme kinetics,Mechanism,Protein labeling

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