A 3D Spheroid Model for Glioblastoma
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS(2020)
Abstract
Two-dimensional (2D) cell cultures do not mimic in vivo tumor growth satisfactorily. Therefore, three-dimensional (3D) culture spheroid models were developed. These models may be particularly important in the field of neuro-oncology. Indeed, brain tumors have the tendency to invade the healthy brain environment. We describe herein an ideal 3D glioblastoma spheroid-based assay that we developed to study tumor invasion. We provide all technical details and analytical tools to successfully perform this assay.
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Key words
Cancer Research,Issue 158,glioblastoma,patient-derived cell,spheroid,invasion,migration,proliferation,in vitro models
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