Molecular epidemiology of dengue fever outbreaks in Bhutan, 2016-2017

Dengue continues to pose a significant public health problem in tropical and subtropical countries. In Bhutan, first outbreak of dengue fever (DF) was reported in 2004 in a southern border town, followed by sporadic cases over the years. In this study, we analysed DF outbreaks that occurred in 3 different places during the years 2016 and 2017. A total of 533 cases in 2016 and 163 in 2017 were suspected of having of DF, where young adults were mostly affected. A total of 240 acute serum specimens collected and analyzed for serotype by nested RT-PCR revealed predominance of serotypes 1 and 2 (DENV-1 and 2). Phylogenetic analysis using envelope gene for both the serotypes demonstrated cosmopolitan genotype which were closely related to strains from India, indicating that they were probably imported from the neighboring country over the past few years. Author summary Endemicity of DENV in some places of southern Bhutan has been established previously. In this study, we analysed outbreaks of DF that occurred in 3 places over a period of 2 years, 1 of which was previously not known to be endemic to DENV. Serum specimens collected from patients suspected of having DF were analyzed in the Royal Centre for Disease Control (RCDC) in Bhutan and in Armed Forces Research Institute of Medical Sciences (AFRIMS), Thailand. DENV-1 and 2 were established as the causes of the outbreaks, also indicating that serotypes of DENV circulating in the country over the past years have remained the same. Our analyses reveal that the current DENV-1 and DENV-2 in Bhutan probably originated from India, Bhutan's closest neighboring country.