Sequence structure character of IgNAR Sec in whitespotted bamboo shark (Chiloscyllium plagiosum)

Whitespotted bamboo shark (Chiloscyllium plagiosum) is a demersal cartilaginous fish with an adaptive immune system founded upon immunoglobulins. In this manuscript, we characterize the IgNAR of the whitespotted bamboo shark. A newly discovered alternative splicing form of IgNAR Sec (IgNAR(sh)(ort) (Delta C2-C3) Sec) was identified, in which the C1 domain was spliced directly to the C4 domain, the process resulted in a molecule containing three constant domains. However, a single unpaired cysteine remains in the highly flexible hinge region, contributing in the formation of an interchain disulfide bond. Two types of C1 domain were found, and the one lacking a short a-helix showed lower proportion. This finding suggests that short alpha-helices might be important to the stability of IgNAR. High-throughput sequencing revealed that the percentage of V-NAR types significantly vary between the diverse species of sharks. The variable region of IgNAR (the V-NAR) with small size and stabilization is a potential candidate for immunotherapeutic agents. The structure and stability analysis in this manuscript may be useful in future biomedical applications.