Method for estimating average grey-level's measurement uncertainty from ultrasound images for non-invasive estimation of temperature in different tissue types.

•Ultrasound is a feasible tool for noninvasive thermometry.•To estimate measurement uncertainty in US noninvasive thermometry stills a challenge.•Average grey-levels calculated from B-Mode images can estimate temperature variations.•Assessing AVGL for different tissues in the same sample leads to uncertainty of 0.68 °C.