[Quantification and fast-screening of malachite green in aquaculture water using paper spray ionization mass spectrometry].

OBJECTIVE:A rapid quantitative technique had been developed for the screening of malachite green(MG) in aquaculture water using paper spray ionization mass spectrometry(PSI-MS). METHODS:To rapid extract MG, chloroform and deep eutectic solvents(DESs) were used as extraction agent and co-solvent respectively. malachite green D_5(MG-D_5) was used as an internal standard to quantitative determination MG, C_(18) chromatographic column(4. 6 mm×250 mm, 5 μm). RESULTS:The method showed a good linearity(R~2=0. 999) in the concentration range of 0. 1-10 μg/mL with the detection limit of 0. 03 μg/mL and the quantitative limit of 0. 1 μg/mL. The intra-and inter-day precisions were 3. 5% and 6. 2%, and the recovery ranged from 98. 9% to 106. 9%(n=7). The detection result by PSI-MS were in agreement with those by liquid chromatography-tandem mass spectrometry(LC-MS/MS), but the run time was only 1 min each sample. CONCLUSION:The method is accurate and reliable. And compared with the conventional LC-MS/MS technology, it is simple, rapid and low-cost.