Development Of In Vitro Transcribed Rna As Positive Control For Laboratory Diagnosis Of Sars-Cov-2 In India
INDIAN JOURNAL OF MEDICAL RESEARCH(2020)
摘要
The WHO has recommended reverse transcriptionpolymerase chain reaction (RT-PCR) for the confirmation of coronavirus disease 2019 (COVID-19) diagnosis. Real-time RT-PCR assays with automated extraction systems are required to process large numbers of specimens. Corman et al1 have reported three real-time RT-PCR assays [based on the RNAdependent RNA polymerase (RdRp) gene, envelope (E) gene and nucleocapsid (N) gene] for detecting beta coronaviruses, including severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)1, and additionally, Chu et al2 have reported two real-time RT-PCR assays based on ORF 1b and N gene that are highly conserved among Sarbeco viruses.
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