Vitamin D Attenuates Loss Of Endothelial Biomarker Expression In Cardio-Endothelial Cells
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES(2020)
Abstract
Vitamin D is associated with cardiovascular health through activating the vitamin D receptor that targets genes related to cardiovascular disease (CVD). The human cardiac microvascular endothelial cells (HCMECs) were used to develop mechanically and TGF-beta 1-induced fibrosis models, and the rat was used as the isoproterenol (ISO)-induced fibrosis model. The rats were injected with ISO for the first five days, followed by vitamin D injection for the consecutive three weeks before being sacrificed on the fourth week. Results showed that mechanical stretching reduced endothelial cell marker CD31 and VE-cadherin protein expressions, as well as increased alpha-smooth muscle actin (alpha-SMA) and fibronectin (FN). The transforming growth factor-beta 1 (TGF-beta 1) reduced CD31, and increased alpha-SMA and FN protein expression levels. Vitamin D presence led to higher protein expression of CD31, and lower protein expressions of alpha-SMA and FN compared to the control in the TGF-beta 1-induced fibrosis model. Additionally, protein expression of VE-cadherin was increased and fibroblast-specific protein-1 (FSP1) was decreased after vitamin D treatment in the ISO-induced fibrosis rat. In conclusion, vitamin D slightly inhibited fibrosis development in cell and animal models. Based on this study, the beneficial effect of vitamin D may be insignificant; however, further investigation of vitamin D's effect in the long-term is required in the future.
MoreTranslated text
Key words
cardiovascular disease,vitamin D,stretching,TGF-beta 1,fibrosis
AI Read Science
Must-Reading Tree
Example
![](https://originalfileserver.aminer.cn/sys/aminer/pubs/mrt_preview.jpeg)
Generate MRT to find the research sequence of this paper
Chat Paper
Summary is being generated by the instructions you defined