ATM is a key driver of NF-κB-dependent DNA-damage-induced senescence, stem cell dysfunction and aging.

NF-kappa B is a transcription factor activated in response to inflammatory, genotoxic and oxidative stress and important for driving senescence and aging. Ataxia-telangiectasia mutated (ATM) kinase, a core component of DNA damage response signaling, activates NF-kappa B in response to genotoxic and oxidative stress via posttranslational modifications. Here we demonstrate that ATM is activated in senescent cells in culture and murine tissues from Ercc1-deficient mouse models of accelerated aging, as well as naturally aged mice. Genetic and pharmacologic inhibition of ATM reduced activation of NF-kappa B and markers of senescence and the senescence-associated secretory phenotype (SASP) in senescent Ercc1(-/-) MEFs. Ercc1(-/Delta) mice heterozygous for Atm have reduced NF-kappa B activity and cellular senescence, improved function of muscle-derived stem/progenitor cells (MDSPCs) and extended healthspan with reduced age-related pathology especially age-related bone and intervertebral disc pathologies. In addition, treatment of Ercc1(-/Delta) mice with the ATM inhibitor KU-55933 suppressed markers of senescence and SASP. Taken together, these results demonstrate that the ATM kinase is a major mediator of DNA damage-induced, NF-kappa B-mediated cellular senescence, stem cell dysfunction and aging and thus, represents a therapeutic target to slow the progression of aging.