Long non-coding RNA MIR4435-2HG recruits miR-802 from FLOT2 to promote melanoma progression.

OBJECTIVE: The regulatory mechanism of lncRNA MIR4435-2HG has been extensively investigated in human cancers other than melanoma. This study aims to elucidate the role of lncRNA MIR4435-2HG in melanoma. MATERIAL AND METHODS: The mRNA expression was detected by RT- qPCR. MTT assay, Transwell assay and Dual-Luciferase reporter assay were used to investigate the regulatory mechanism of lncRNA MIR4435-2HG. RESULTS: Upregulation of lncRNA MIR44352HG was identified in melanoma and promoted melanoma cell proliferation, migration and invasion. In addition, lncRNA MIR4435-2HG serves as the ceRNA of miR-802. MiR-802 inhibited melanoma progression by downregulating lncRNA MIR4435-2HG. Besides, miR-802 directly targets FLOT2. And knockdown of FLOT2 restrained the progression of melanoma by downregulating lncRNA MIR4435-2HG and upregulating miR-802. CONCLUSIONS: LncRNA MIR4435-2HG promotes cell proliferation, migration and invasion in melanoma by sponging miR- 802 and upregulating FLOT2.