Sensitization pattern to Canis familiaris. Optimizing a more potent and patient specific allergenic extract

Dog allergens are a common cause of allergic sensitization and triggering respiratory symptoms worldwide. The knowledge of the composition of the dog epithelia and the structure of the allergens that compose it is a prerequisite to carry out more accurate diagnosis and treatment. A group of 30 patients with a history of moderate rhinitis and/or asthma to dog epithelia were selected. SIgE for conventional dog extract and the respective recombinant molecular components Can f 1, Can f 2, Can f 3, Can f 5, and Can f 6 were evaluated by the ImmunoCAP® method according to obtain the prevalence and serodominance of the selected group. The allergenic profile of each patient was also analysed by western blot using the serum of each of the selected patients and an allergenic extract from a mixture of organic sources (saliva, urine and dandruff) that contains all the allergens described. Twenty-one out 30 patients with moderate or severe asthma shown a pattern of sensitization (mean value) to Dogś allergens, Can f 1, Can f 2, Can f 3, Can f 5, and Can f 6 was 1.5, 0.05, 2.2, 8.6 and 0.1 respectively. Western Blot revealed similar profiles, with relevance for the detection of Can f 5 in 51% (n=16) of patients. The use of various molecular components and complete allergenic extracts containing all the allergens described so far increases the accuracy of the diagnosis used especially in those patients sensitized to proteins presents at low concentrations.