Adhesion and Secretory Profile of Mesenchymal Stem Cells Upon Contact with Some Biomaterials

The aim of this study was to investigate the adhesion and secretory profile of mesenchymal stem cells in contact with biomaterials used in the field of tissue engineering. Mesenchymal stem cells obtained from lipoaspirate and two biomaterials were used: anodized titanium plates coated with hydroxyapatite crystals and collagen meshes crosslinked with formic anhydride combined with poly(epsilon-caprolactone). Cell adhesion capacity of the two types of biomaterials was assessed by counting non-adhering cells in the supernatant, and the secretory profile by ELISA dosing of a series of cytokines and growth factors released by the cells upon contact with the two biomaterials. Faster fixation of mesenchymal stem cells on titanium matrices compared to the collagen ones was observed. In contact with the materials, the mesenchymal stem cells alter their secretory phenotype, with a slight increase in their pro-inflammatory potential.