SpyTag/SpyCatcher cyclization and covalent immobilization in enhancing cephalosporin C acylase stability

•Stability of cephalosporin C acylase was improved using SpyTag/SpyCatcher system.•Multipoint and oriented immobilization was achieved on the glyoxyl agarose carriers.•The immobilization and activity recovery rates were significantly improved.•Stability of immobilized enzyme via SpyTag/SpyCatcher chemistry was greatly improved.