18S-V9 DNA metabarcoding detects the effect of water-quality impairment on stream biofilm eukaryotic assemblages
Ecological Indicators(2020)
Abstract
•DNA metabarcoding is an emergent tool for biodiversity assessments.•Benthic eukaryotic assemblages were characterized using18S-V9 rDNA.•Diatoms, water molds, holozoans, green algae, fungi responded to stream impairment.•This approach may be recommended for biomonitoring of stream eukaryotic biota.
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Key words
Amplicon sequencing,Benthos,Bioassessment,DNA metabarcoding,Eukaryotes,Streams
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