3D Printing in analytical sample preparation

In the last 5 years, additive manufacturing (three-dimensional printing) has emerged as a highly valuable technology to advance the field of analytical sample preparation. Three-dimensional printing enabled the cost-effective and rapid fabrication of devices for sample preparation, especially in flow-based mode, opening new possibilities for the development of automated analytical methods. Recent advances involve membrane-based three-dimensional printed separation devices fabricated by print-pause-print and multi-material three-dimensional printing, or improved three-dimensional printed holders for solid-phase extraction containing sorbent bead packings, extraction disks, fibers, and magnetic particles. Other recent developments rely on the direct three-dimensional printing of extraction sorbents, the functionalization of commercial three-dimensional printable resins, or the coating of three-dimensional printed devices with functional micro/nanomaterials. In addition, improved devices for liquid-liquid extraction such as extraction chambers, or phase separators are opening new possibilities for analytical method development combined with high-performance liquid chromatography. The present review outlines the current state-of-the-art of three-dimensional printing in analytical sample preparation.