A platform for context-specific genetic engineering of recombinant protein production by CHO cells.

•We demonstrate a platform for high-throughput testing of functional genes.•Genetic effectors of the secretory pathway can be used to improve recombinant protein production.•Thorough assessment of gene combinations can lead to greaterimprovements in productivity than single genes alone.•Transient and stable production of the same molecule is improved by different genetic effectors.