Effects of microcarriers addition and mixing on WJ-MSC culture in bioreactors

C. Sion, C. Loubière,M.K. Wlodarczyk-Biegun, N. Davoudi, C. Müller-Renno, E. Guedon,I. Chevalot,E. Olmos

Biochemical Engineering Journal（2020）

引用 8|浏览22

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摘要

•New strategy of controlled addition of microcarriers to improve WJ-MSC expansion.•Cell quality was not impacted by bead-to-bead transfer ability of WJ-MSC.•Orbital agitation led to a significant increase of cell death.•Mechanical agitation at Njs was more suitable for WJ-MSC expansion.

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关键词

Umbilical cord mesenchymal stem cells,Bead-to-bead transfer,Shocks and collisions,Cell growth,Bioreactor,Agitation

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