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A Single Tube Overlap Extension PCR Method for Splicing of Multiple DNA Fragments.

Farzaneh Zarghampoor, Abbas Behzad-Behbahani, Negar Azarpira, Saeed Reza Khatami, Maryam Fanian, Mahdokht Hossein Aghdaie, Gholamreza Rafiei Dehbidi

Avicenna journal of medical biotechnology（2020）

Cited 25|Views10
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Abstract
Single tube SOEing PCR is a valuable method to construct more multiple fragments with high yield. The method can successfully be applied for construction of various kinds of complex chimeric genes.
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Key words
Beta-globins,Mutagenesis,Polymerase chain reaction,Site-directed,Untranslated regions
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