Propagation of jundia Rhamdia quelen (Siluriformes: Heptapteridae) by applying the ovarian sperm injection method

We compared the effectiveness of the ovarian sperm injection method with that of the traditional one over the artificial propagation of jundia. Three experimental groups were set up containing seven females each. Females in Group 1 received common carp pituitary extract (CPE) homogenized in saline to induce ovulation and the stripped eggs were fertilized with the traditional dry method. In Group 2 ovulation was induced as in Group 1, however, sperm for fertilization was injected to the ovarian cavity of the females. In Group 3, CPE was first homogenized in sperm which was then injected to the ovarian cavity of females. All females received the same dose of 5.0 mg kg(-1) bodyweight. Females in Group 2 and 3 were inseminated by a catheter. To fertilize the eggs, sperm samples were pooled from six males and they were used for all groups. There were no significant differences among the three Groups (P < 0.05) in the investigated parameters, such as ovulation ratio, pseudo-gonadosomatic index and fertilization rate. Our results demonstrated that the effectiveness of the sperm injection method was similar to that of the traditional one.