The complete mitochondrial genome of the brown pansy butterfly, Junonia stygia (Aurivillius, 1894), (Insecta: Lepidoptera: Nymphalidae)

Seun Ajibola,Vibhuti Arya, Emily N. Barker,Kirsten T. Biggar, Dominic M. Bohemier, Julina N. Braga, Jessica L. Buchel, Vicky Bui,Julian M. Burtniak,Codey E. Dueck,Steven J. Dupas,Shayna J. Giesbrecht, Alexandra Haverstick, Stefan B. Hreno,Amy L. Irvine, Carter Johnson, Ivory C. Jorgenson, Matthew R. Kroeker, Corrine M. Kuo, Joohee Lee, Vatineh N. Magaji, Gillian J. McIvor, Katrina S. Melgarejo, Michael D. Moore,Olamide U. Ogungbola,Josephine E. Payment, Daniel O. Peter-Salawu, Ashton P. Raitt,Breann T. Recksiedler, Megan Rodriguez, Rahel B. Sahlemariam, Shabadjot Sandhawalia, Mackenzie A. Sarvis, Megan L. Skakum, Jordan C. Small, Kassandra R. Taverner, Chaltu B. Tesfaye,Lea J. Tessier, Catherine J. Unrau, Natasha G. M. Wadlow,Jeffrey M. Marcus

MITOCHONDRIAL DNA PART B-RESOURCES（2020）

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摘要

The brown pansy, Junonia stygia (Aurivillius, 1894) (Lepidoptera: Nymphalidae), is a widespread West African forest butterfly. Genome skimming by Illumina sequencing allowed assembly of a complete 15,233 bp circular mitogenome from J. stygia consisting of 79.5% AT nucleotides. Mitochondrial gene order and composition is identical to other butterfly mitogenomes. Junonia stygia COX1 features an atypical CGA start codon, while ATP6, COX1, COX2, ND4, and ND4L exhibit incomplete stop codons. Phylogenetic reconstruction supports a monophyletic Subfamily Nymphalinae, Tribe Junoniini, and genus Junonia. The phylogenetic tree places Junonia iphita and J. stygia as basal mitogenome lineages sister to the remaining Junonia sequences.

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关键词

Illumina sequencing,mitogenomics,inquiry-based learning,Lepidoptera,Nymphalidae

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