The Effect of TISSEEL? on Colorectal Anastomosis Healing Process in a Diabetic Animal Experimental Model

Background/Aim: Diabetes mellitus is an established risk factor of colorectal anastomosis failure. The purpose of the present study was to evaluate the effect of TISSEEL (R) in anastomotic healing. Materials and Methods: Forty male, Sprague-Dawley rats were used. Diabetes was induced in half of them by intraperitoneal injection of Streptozotocin, 60 mg/kg. One week after the injection, animals were operated and a 1 cm segment was removed and an end-to-end hand sewn anastomosis was performed. TISSEEL (R) was applied in each group (diabetic, non-diabetic) following randomization. Results: The pathology analysis revealed improved tissue remodeling in the TISSEEL (R) group, both for the normoglycemic and the diabetic group. Specifically, the extent of inflammation was decreased (p<0.001), whereas fibroblast and collagen formation were improved (p=0.040 and p=0.008). Neovascularization was also improved (p=0.047). Conclusion: Application of TISSEEL (R) on colorectal anastomoses improves healing in rats that suffer from severe hyperglycemia.