Effect of ScLL and 15d-PGJ₂ on viability and cytokine release in LPS-stimulated fibroblasts: an in vitro study

This study evaluated the effect of a cyclopentenone-type PG, 15-Deoxy-(Delta)(12)(,14)-p G J2 (15d-PGJ(2)), and lectin (ScLL) on the viability of human gingival fibroblasts (HGFs), and on IL-6 and TGF beta-1 release by these fibroblasts, stimulated with lipopolysaccharide (LPS). HGFs were stimulated with LPS 10 mu g/ml and treated with 15d-PGJ(2)1 and 2 mu g/ml, and ScLL 2 and 5 mu g/ml, for 1 and 3h, and then evaluated for viability by MTT assay. Supernatant was collected to detect IL-6 and TGF beta-1 release, by ELISA. Positive control was cells kept in Dulbecco's Modified Eagle's Medium, and negative control was those kept in LPS. Data were analyzed by ANOVA and Dunnett's test (alpha = 0.05). No significant difference was found in viability among experimental groups at 1h (p > 0.05). Percentage of ScLL 5 mu g/ml viable cells was similar to that of positive control at evaluated periods (p > 0.05), whereas the other groups had lower levels than the positive control (p < 0.05). IL-6 release was statistically higher for ScLL 5 mu g/ml and 15d-PGJ(2) 2 mu g/ml at 1h, compared with the other treated groups and positive control (p < 0.05). No significant differences were found among the groups at 3h (p > 0.05), except for ScLL 2 mu g/ml and 15d-PGJ(2) 1 mu g/ml, which showed lower IL-6 release compared with that of negative control (p < 0.05). No significant difference was found among the groups for TGF beta-1 release (p > 0.05). Results indicated that ScLL 5 mu g/ml did not interfere in viability, and ScLL 2 mu g/ml and 15d-PGJ(2) 1 mu g/ml demonstrated reduced IL-6 release. Tested substances had no effect on TGF beta-1 release.