Very Rapid Cloning, Expression And Identifying Specificity Of T-Cell Receptors For T-Cell Engineering

Neoantigens can be predicted and in some cases identified using the data obtained from the whole exome sequencing and transcriptome sequencing of tumor cells. These sequencing data can be coupled with single-cell RNA sequencing for the direct interrogation of the transcriptome, surfaceome, and pairing of alpha beta T-cell receptors (TCR alpha beta) from hundreds of single T cells. Using these 2 large datasets, we established a platform for identifying antigens recognized by TCR alpha beta s obtained from single T cells. Our approach is based on the rapid expression of cloned TCR alpha beta genes as Sleeping Beauty transposons and the determination of the introduced TCR alpha beta s' antigen specificity and avidity using a reporter cell line. The platform enables the very rapid identification of tumor-reactive TCRs for the bioengineering of T cells with redirected specificity.