Cellulomonas Shaoxiangyii Sp. Nov., Isolated From Faeces Of Tibetan Antelope (Pantholops Hodgsonii) On The Qinghai-Tibet Plateau
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY(2020)
摘要
Two Gram-stain-positive, catalase-positive and oxidase-negative, aerobic, non-motile, cellobiose-utilizing, short-rodshaped strains (Z28(T) and Z29) were isolated from faeces of Tibetan antelope (Pantholops hodgsonii) collected on the Qinghai-Tibet Plateau. Strain Z28(T) shared 98.1, 98.0, 97.8 and 97.4% 16S rRNA gene similarity, 24.1, 22.8, 23.2 and 26.3% digital DNA-DNA hybridization relatedness and 80.8, 80.0, 80.7 and 80.9% average nucleotide identity values with Cellulomonas oligotrophica DSM 24482(T), Cellulomonas flavigena DSM 20109(T), Cellulomonas iranensis DSM 14785(T) and Cellulomonas terrae JCM 14899(T), respectively. Results from further phylogenetic analyses based on the 165 rRNA gene and 148 core genes indicated that strains Z28(T) and Z29 were closest to C. oligotrophica DSM 24482(T) and C. flavigena DSM 20109(T), but clearly separated from the currently recognized species of the genus Cellulomonas. The genomic DNA G+C content of strain Z281 was 75.3 mol%. The major cellular fatty acids were anteiso-C-15:0, anteiso-C-15:1 A, C-16:0 and anteiso-C-17:0. Ribose and mannose were detected as the whole-cell sugars. The major respiratory quinone was MK-9(H4) and ornithine was the diamino acid of the cell wall. The polar lipids present in strain Z28(T) were phosphatidylethanolamine, five phospholipids, two aminophospholipids, aminolipid and three unidentified lipids. Comparison of phenotypic and phylogenetic features between the two strains and the related organisms revealed that Z28(T) and Z29 represent a novel species of the genus Cellulomonas, for which the name Cellulomonas shaoxiangyii sp. nov. is proposed. The type strain is Z28(T) (=CGMCC 1.16477(T)=DSM 106200(T)).
更多查看译文
关键词
Cellulomonas shaoxiangyii, Qinghai-Tibet Plateau, Tibetan antelopes (Pantholops hodgsonii)
AI 理解论文
溯源树
样例
生成溯源树,研究论文发展脉络