An optimized protocol for high yield expression and purification of an extremophilic protein
PROTEIN EXPRESSION AND PURIFICATION(2020)
Abstract
•High yield expression and purification of extremophilic enzymes in a mesophilic expression host.•The target is extremophilic lytic transglycosylase from Beebe hydrothermal vent plume, depth 4316 m, Mid Cayman Rise.•pI could be used as guide for pH of the buffers used in protein extraction and the binding metal in the purification matrix.
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