A novel rat model of inflammatory bowel disease developed using a device created with a 3D printer

•Adipose-derived stem cells (ASCs) are being explored as a new treatment for IBD because they can downregulate inflammation and improve tissue repair.•A new model of TNBS-induced colitis was developed using a custom-designed device fabricated by a 3D printer.•The novel model of colitis generated ulcers that were highly reproducible in size.•The size of the ulcer was reduced by mesalazine or prednisolone (administered as an enema) or by localized injection of ASCs.