Detection of Optimal Puncture Position in OVUM Images for Artificial Insemination

This paper aims to determine the optimal puncture position of ovum by evaluating rupture membrane of cytoplasm. We employed 139 ovum images on the Piezo-ICSI (Intracytoplasmic sperm injection). In it, grayscale images before puncture and their actual puncture position were obtained from the movie file (Rupture:31, No Rupture:108), and Local Binary Pattern (LBP) feature is calculated at analysis area around the puncture position. LBP feature dimensions are reduced, and data are classified by hierarchical clustering method using feature of three dimensions. As a result, the data classified into two clusters (Clusters A and B). Cluster A has 7 Ruptures and 50 No Ruptures, Cluster B has 24 Ruptures and 58 No Ruptures. Then, the sensitivity is 0.77. Therefore, it is possible to evaluate rupture membrane of cytoplasm from shape feature of membrane. The optimal puncture position could be determined by the features.