Mir-505 Functions As A Tumor Suppressor In Glioma By Targeting Insulin Like Growth Factor 1 Receptor Expression

MicroRNAs (miRNAs) are small non-coding RNA molecules that regulate gene expression at the post-transcriptional level. Compelling evidence shows that there are causative links between miRNAs abnormal regulation and the development of cancer. miR-505 has been reported to be aberrant expression and functions as a tumor suppressor in many human cancers, but its roles and potential molecular mechanism in glioma remain unclear. Here, we found that the expression levels of miR-505 were down-regulated in glioma tissues and cell lines. Exogenous over-expression of miR-505 resulted in inhibited cell proliferation and invasion in glioma in vitro. Furthermore, dual luciferase reporter assay and western blot analysis confirmed that IGF1R (Insulin like growth factor 1 receptor) was a direct target gene of miR-505 in glioma. More importantly, over-expression of IGF1R rescued miR-505-mediated inhibition of cell proliferation and invasion in glioma in vitro. Taken together, our results suggest that miR-505 acts as a tumor suppressor in glioma via direct negative regulation of IGF1R, which may provide a novel therapeutic strategy.