Data for high-throughput screening of enzyme mutants by comparison of their activity ratios to an enzyme tag.
Data in brief(2020)
摘要
Data in this article are associated with the research article "High-throughput screening of enzyme mutants by comparison of their activity ratios to an enzyme tag" (Li et al., 2019) [1]. Data are provided on the development of a system for high-throughput (HTP) screening of mutants through the comparison of the activity ratios of an applicable enzyme and its mutants to a suitable tag enzyme in cell lysates of their fused forms, with alkaline phosphatase (ECAP) as the tag fused to the -terminus of arylsulfatase (PAAS) and its mutants a flexible linker. Data were made publicly available for further analyses.
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关键词
Activity ratio,Enzyme tag,Fusion expression,HTP screening,Mutant libraries
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