Osseointegrated membranes based on electro-spun TiO2/hydroxyapatite/polyurethane for oral maxillofacial surgery

Membranes which have an osseointegration abilty are often selected as biomaterials in oral and maxillofacial surgery. Although these membranes are often the best option for certain uses, it is a challenge to create functionally attractive membranes. In this research, electro-spun titanium oxide (TiO2)/hydroxyapatite (HA)/polyurethane (PU) membranes were fabricated with different ratios of HA and TiO2: 100: 0, 70:30, 50:50, 30:70 and 0:100 w/w. The morphologies of the different mixtures were assessed with a Scanning Electron Microscope (SEM) and Field Emission Microscope (FESEM). Element analysis was performed with EDX. The physical properties of the water contact angles and mechanical strength were tested and the membranes cultured with osteoblasts to evaluate their biological functions, cell adhesion, viability, proliferation, alkaline phosphatase (ALP) activity, and calcium content. The results showed that the membranes with TiO2 and HA had smaller fibers than those without TiO2 and HA. The TiO2- and HA-including compounds showed the formation of particle aggregation on the surface of the fibers. They also had higher water contact angles, mechanical strength, and stiffness than those without TiO2 and HA, and they had better cell adhesion, viability, proliferation, ALP activity and calcium content. The membrane with a 50:50 TiO2:HA ratio had more unique biological functions than the others. Finally, our research demonstrated that osseointegrated membranes with 50:50 TiO2:HA are promising for oral and maxillofacial surgery.