Molecular Detection of Group A Rotaviruses from the Bhandara and Chandrapur District of Maharashtra State

Animal Rotaviruses (RVs) are considered as major pathogen threats to humans and livestock due to neonatal mortality and possibility of interspecies transmission and exchange of genomic materials from human to animal species and vice versa. There are eight groups of rotaviruses (A to H), among them mostly Group A mostly causes diarrhea in neonates and infants in humans and animals especially in cattle and buffalo worldwide. The present study was carried out to emphasize for molecular detection of circulating rotaviruses in Bhandara and Chandrapur districts of Maharashtra state in India. Ninety two (92) fecal samples collected from diarrheic and non diarrheic bovine calves (39 cow calves and 53 buffalo calves) were analyzed by conventional latex agglutination test (LAT) and amplification of VP6,VP7 and VP4 gene by RT-PCR and further analysis by G and P Typing PCR. From ninety two samples, ten samples were found positive by LAT, out of ten LAT positive samples, four samples showed expected 309 bp amplicon for group specific VP6 gene. The VP4 and VP7 RT-PCR showed 864 bp and 1062 bp amplicons specific for VP4 and VP7 respectively in same two fecal out of ten positive samples. The G-typing PCR showed the G10 type strain is circulating whereas P type was untypable.Hence the present study concludes, the presence of group A Rotaviruses of G10 Type in Bhandara district and untypable rotaviruses in Chandrapur district of Vidharbha region of Maharashtra state in India.