N-terminal sequences affect expression of triterpene biosynthesis enzymes in Chlamydomonas chloroplasts
Algal Research(2019)
Abstract
•Several cassettes were designed for algal chloroplast expression of botryococcenes.•Transcript accumulation leads to highly variable protein accumulation.•Altering the amino terminal of foreign proteins could increase protein abundance.•Post-translational regulation is therefore a key consideration in Chlamydomonas.
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Key words
Chloroplast,Protein expression,N-terminal sequence,Algae,Chlamydomonas reinhardtii,Botryococcene
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