Synthesis of NickFects, a New Family of CPPs, by Solid-Phase Peptide Synthesis.

Cell-penetrating peptides (CPPs) are relatively short peptides that can enter to the cell interior and facilitate intracellular delivery of associated cargo molecules. NickFects is a novel family of CPPs, designed to deliver various types of bio-active cargos using non-covalent nanoparticle formation approach. This chapter describes in details the manual synthesis of cell-penetrating peptides using Fmoc-solid phase peptide synthesis (SPPS).