Preparation monoclonal -type anti-idiotype antibody of zearalenone and development of green ELISA quantitative detecting technique

Immunoassay has been widely used in the screening of mycotoxins, which may be hazardous to the operator or the environment. This study was to develop a green way to measure zearalenone (ZEN) with a monoclonal beta-type anti-idiotype antibody (Ab2 beta) against ZEN in place of ZEN standard. Six monoclonal beta-type anti-idiotype antibodies were prepared. The 50% inhibitory concentration (IC50) value to ZEN of the six antibodies was between 34.45 +/- 1.12-182.12 +/- 15.40 nM. A green ELISA was then developed and validated. The quantitative conversion formula between ZEN and the monoclonal Ab2 beta against ZEN was y = 0.092x(0.722), R-2 = 0.990. The working range was 2.63-100.64 ng ml(-1). The recovery rate in spiked feed samples was from 82.15% to 102.79%, and the within-assay and between-assay coefficient variation (CV) level were less than 10.00%. A good correlation was obtained by high-performance liquid chromatography method (HPLC) to validate the developed method.