A spatially-resolved approach to visualize the distribution and biosynthesis of flavones in Scutellaria baicalensis Georgi.

•Flavones, glycosides, carbohydrates, phenolic acids were imaged in S. baicalensis.•Spatially-resolved metabolic network of flavone and their glycoside was visualized.•Region-specific activities of enzymes in flavone synthesis pathway were detected.•Spatial feature of metabolite and enzyme in flavone synthesis pathway were compared.