Stanazolol derived ELISA as a sensitive forensic tool for the detection of multiple 17α-methylated anabolics.

Lukáš Huml,Dominika Havlová,Ondřej Longin,Eliška Staňková,Barbora Holubová,Martin Kuchař, Elena Prokudina,Zdeňka Rottnerová,Tomáš Zimmermann,Pavel Drašar,Oldřich Lapčík,Michal Jurášek

Steroids（2020）

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摘要

•Stanazolol antigens and polyclonal rabbit antibodies (RAbs) were produced.•Biotinylated antigens were synthesized.•Two highly stable ELISA methods were developed.•Sensitivity towards multiple 17α-methylated anabolics was identified.•Developed methods were applied for real samples analyses.

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关键词

AAS,ST,ELISA,AB-ELISA,RSA,BSA,CR,PEG,RAb,RT

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