Marinobacter Fonticola Sp. Nov., Isolated From Deep Sea Cold Seep Sediment

In this study, we report a novel Gram-negative bacterium, designated as strain CS412(T), isolated from deep-sea sediment collected in a cold seep area of the South China Sea. Growth of strain CS412(T) occurred at 4-40 degrees C (optimum, 28 degrees C). pH 5.0-11.0 (optimum, pH 6.0) and with 0-19% (w/v) NaCl (optimum, 1-2%). Phylogenetic analysis based on 16S rRNA gene sequence data indicated that strain CS412(T) belonged to the genus Marinobacter. The closest phylogenetic neighbours of strain CS412(T) were Marinobacter pelagius HS225(T) (96.9%), Marinobacter szutsaonensis NTU-104(T) (96.8%), Marinobacter santoriniensis NKSG1(T) (96.4%) and Marinobacter koreensis ob-Mar (96.3%). The genomic DNA G+C content of strain CS412(T) was 58.0 mol%. The principal respiratory quinone was ubiquinone-9 (Q-9). The polar lipids of CS412(T) contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, aminophospholipidand and four glycolipids. The major fatty acids of CS412(T) contained cyclo-C-19:0 omega 8c, C-16:0, C-18:1 omega 7c 11-methyl. The results of phylogenetic, physiological, biochemical and morphological analyses suggested that strain CS412(T) represents a novel species of the genus Marinobacter, and the name Marinobacter fonticola sp. nov. is proposed with the type species CS412(T) (=CCTCC AB 2019197(T)=KCTC 72475(T)).