A ratiometric fluorescent probe for simultaneous sensing cysteine/homocysteine and glutathione in living cells and zebrafish

Based on FRET mechanism, a ratiometric fluorescent probe, CPR, was constructed to simultaneously distinguish GSH and Cys/Hcy by means of multiple emissions. This probe showed high sensitivity and selectivity with low limits of detection and was capable of imaging GSH and Cys/Hcy in cells and zebrafish with low toxicity.